EIF4B antibody

Catalog No. ABIN7220186

The Rabbit Polyclonal anti-EIF4B antibody (ABIN7220186) specifically detects EIF4B in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for EIF4B antibody (ABIN7220186)

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-EIF4B Antibody

Purpose: Rabbit Anti-eIF4B Polyclonal Antibody

Specificity: EIF4B Polyclonal Antibody detects endogenous levels of eIF4B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human eIF4B around the non-phosphorylation site of S422

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EIF4B

Target: EIF4B (Eukaryotic Translation Initiation Factor 4B (EIF4B))

Alternative Name: eIF4B

Background: EIF4B, Eukaryotic translation initiation factor 4B, eIF-4BRequired for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Molecular Weight: 70kD

Gene ID: 1975

UniProt: P23588