EIF4E antibody (pSer209)

Catalog No. ABIN7212948

The Rabbit Polyclonal anti-EIF4E antibody is suitable to detect EIF4E in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for EIF4E antibody (pSer209) (ABIN7212948)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-EIF4E Antibody

Binding Specificity: pSer209

Purpose: Rabbit Anti-eIF4E (phospho Ser209) Polyclonal Antibody

Specificity: Phospho-eIF4E (S209) Polyclonal Antibody detects endogenous levels of eIF4E protein only when phosphorylated at S209.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human eIF4E Phospho-Ser209

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: eIF4E

Background: EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding proteinEukaryotic translation initiation factor 4E encoded by EIF4E is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs.Eukaryotic translation initiation factor 4E aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. EIF4E acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of EIF4E are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Molecular Weight: 25kD

Gene ID: 1977

UniProt: P06730

Pathways: BCR Signaling