EIF5B antibody (AA 1020-1100)

Catalog No. ABIN7226064

The Rabbit Polyclonal anti-EIF5B antibody (ABIN7226064) specifically detects EIF5B in WB, ELISA, IF and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EIF5B antibody (AA 1020-1100) (ABIN7226064)

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF5B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-EIF5B Antibody

Binding Specificity: AA 1020-1100

Purpose: Rabbit Anti-eIF5B Polyclonal Antibody

Specificity: EIF5B Polyclonal Antibody detects endogenous levels of eIF5B protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human eIF5B at AA range: 1020-1100

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EIF5B

Target: EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))

Alternative Name: eIF5B

Background: EIF5B, IF2, KIAA0741, Eukaryotic translation initiation factor 5B, eIF-5B, Translation initiation factor IF-2Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B (eukaryotic translation initiation factor 5B). The gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

Molecular Weight: 139kD

Gene ID: 9669

UniProt: O60841