Factor I antibody (AA 410-490)

Catalog No. ABIN7221664

The Rabbit Polyclonal anti-Factor I antibody is suitable to detect Factor I in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for Factor I antibody (AA 410-490) (ABIN7221664)

Target: Factor I

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Factor I antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Factor I Antibody

Binding Specificity: AA 410-490

Purpose: Rabbit Anti-Factor I Polyclonal Antibody

Specificity: Factor I Polyclonal Antibody detects endogenous levels of Factor I protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Factor I at AA range: 410-490

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Factor I

Target: Factor I

Background: CFI, IF, Complement factor I, C3B/C4B inactivatorCFI encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene.

Molecular Weight: Full lenth:66kD,heavy chain:50-58kD

Gene ID: 3426

UniProt: P05156