Coagulation Factor X antibody (Cleaved-Ala41)

Catalog No. ABIN7212514

This Rabbit Polyclonal antibody specifically detects Coagulation Factor X in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Coagulation Factor X antibody (Cleaved-Ala41) (ABIN7212514)

Target: Coagulation Factor X (F10)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Coagulation Factor X antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Coagulation Factor X Antibody

Binding Specificity: AA 370-450, Cleaved-Ala41

Purpose: Rabbit Anti-Cleaved-Factor X/ Factor X LC (A41) Polyclonal Antibody

Specificity: Cleaved-Factor X/ Factor X LC (A41) Polyclonal Antibody detects endogenous levels of fragment of activated Factor X/ Factor X LC protein resulting from cleavage adjacent to A41.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Factor X/ Factor X LC at AA range: 370-450

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Coagulation Factor X

Target: Coagulation Factor X (F10)

Alternative Name: Factor X/ Factor X LC

Background: F10, Coagulation factor X, Stuart factor, Stuart-Prower factorF10 encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds, the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides.

Molecular Weight: 50kD

Gene ID: 2159

UniProt: P00742