Flk-1/Flt-4 (pTyr1054) antibody

Catalog No. ABIN7222030

The Rabbit Polyclonal anti-Flk-1/Flt-4 antibody is suitable to detect Flk-1/Flt-4 in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC and IF.

Quick Overview for Flk-1/Flt-4 (pTyr1054) antibody (ABIN7222030)

Target: Flk-1/Flt-4

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Binding Specificity: pTyr1054

Purpose: Rabbit Anti-Flk-1/Flt-4 (phospho Tyr1054/Y1063) Polyclonal Antibody

Specificity: Phospho-Flk-1/Flt-4 (Y1054/Y1063) Polyclonal Antibody detects endogenous levels of Flk-1/Flt-4 protein only when phosphorylated at Y1054/Y1063.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Flk-1/Flt-4 Phospho-Tyr1054/Y1063

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Flk-1/Flt-4

Background: KDR, FLK1, VEGFR2, Vascular endothelial growth factor receptor 2, VEGFR-2, Fetal liver kinase 1, FLK-1, Kinase insert domain receptor, KDR, Protein-tyrosine kinase receptor flk-1, CD antigen CD309, FLT4, VEGFR3, Vascular endothelial growthVEGF R1 is one of the five receptor tyrosine kinases (RTKs) (Flt-1, KDR/Flk-1, Flt-4, tie-1, and tek/tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. Flt-1, KDR/Flk-1 and Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Gene ID: 3791, 2324

UniProt: P35968, P35916