FOXO4 antibody (pSer197)

Catalog No. ABIN7212968

The Rabbit Polyclonal anti-FOXO4 antibody has been validated for WB, ELISA and IHC (p). It is suitable to detect FOXO4 in samples from Human and Mouse.

Quick Overview for FOXO4 antibody (pSer197) (ABIN7212968)

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXO4 Antibody

Binding Specificity: pSer197

Purpose: FoxO4 (phospho Ser197) Polyclonal Antibody

Specificity: Phospho-FoxO4 (S197) Polyclonal Antibody detects endogenous levels of FoxO4 protein only when phosphorylated at S197.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human FoxO4 Phospho-Ser197

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for FOXO4

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Alternative Name: FoxO4

Background: Rabbit Anti-FoxO4 (phospho Ser197) Polyclonal Antibody,FOXO4, AFX, AFX1, MLLT7, Forkhead box protein O4, Fork head domain transcription factor AFX1,FOXO4 encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving FOXO4 on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein (forkhead box O4), located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for FOXO4.,Forkhead box protein O4

Molecular Weight: observerd band 70kDa

Gene ID: 4303

UniProt: P98177

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation