FLI1 antibody (AA 260-340)

Catalog No. ABIN7214910

The Rabbit Polyclonal anti-FLI1 antibody (ABIN7214910) specifically detects FLI1 in WB, ELISA, IHC and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for FLI1 antibody (AA 260-340) (ABIN7214910)

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-FLI1 Antibody

Binding Specificity: AA 260-340

Purpose: Rabbit Anti-Fli-1 Polyclonal Antibody

Specificity: Fli-1 Polyclonal Antibody detects endogenous levels of Fli-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Fli-1 at AA range: 260-340

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FLI1

Target: FLI1 (Friend Leukemia Virus Integration 1 (FLI1))

Alternative Name: Fli-1

Background: FLI1, Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGBFLI1 encodes friend leukemia integration 1 transcription factor containing an ETS DNA-binding domain. FLI1 can undergo a t (11,22) (q24,q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t (4,11) (q21,q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants.

Molecular Weight: 50kD

Gene ID: 2313

UniProt: Q01543