FZD5 antibody (AA 440-520)

Catalog No. ABIN7225030

The Rabbit Polyclonal anti-FZD5 antibody is suitable to detect FZD5 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for FZD5 antibody (AA 440-520) (ABIN7225030)

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-FZD5 Antibody

Binding Specificity: AA 440-520

Purpose: Rabbit Anti-Frizzled-5 Polyclonal Antibody

Specificity: Frizzled-5 Polyclonal Antibody detects endogenous levels of Frizzled-5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Frizzled-5 at AA range: 440-520

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FZD5

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Alternative Name: Frizzled-5

Background: FZD5, C2orf31, Frizzled-5, Fz-5, hFz5, FzE5Members of the frizzled gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 (frizzled class receptor 5) protein is believed to be the receptor for the Wnt5A ligand.

Molecular Weight: 65kD

Gene ID: 7855

UniProt: Q13467

Pathways: WNT Signaling