FZD6 antibody (AA 90-170)

Catalog No. ABIN7225244

This Rabbit Polyclonal antibody specifically detects FZD6 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for FZD6 antibody (AA 90-170) (ABIN7225244)

Target: FZD6 (Frizzled Family Receptor 6 (FZD6))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-FZD6 Antibody

Binding Specificity: AA 90-170

Purpose: Rabbit Anti-Frizzled-6 Polyclonal Antibody

Specificity: Frizzled-6 Polyclonal Antibody detects endogenous levels of Frizzled-6 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Frizzled-6 at AA range: 90-170

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FZD6

Target: FZD6 (Frizzled Family Receptor 6 (FZD6))

Alternative Name: Frizzled-6

Background: FZD6, Frizzled-6, Fz-6, hFz6FZD6 (frizzled class receptor 6) represents a member of the frizzled gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.

Molecular Weight: 79kD

Gene ID: 8323

UniProt: O60353

Pathways: WNT Signaling, Tube Formation