Frizzled5/8 antibody (AA 10-90)

Catalog No. ABIN7225248

The Rabbit Polyclonal anti-Frizzled5/8 antibody (ABIN7225248) specifically detects Frizzled5/8 in WB, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Frizzled5/8 antibody (AA 10-90) (ABIN7225248)

Target: Frizzled5/8 (HYFZD5/8)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Frizzled5/8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-Frizzled5/8 Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-Frizzled-5/8 Polyclonal Antibody

Specificity: Frizzled-5/8 Polyclonal Antibody detects endogenous levels of Frizzled-5/8 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Frizzled-5/8 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Frizzled5/8

Target: Frizzled5/8 (HYFZD5/8)

Alternative Name: Frizzled-5/8

Background: FZD5, C2orf31, Frizzled-5, Fz-5, hFz5, FzE5, FZD8, Frizzled-8, Fz-8, hFz8This intronless gene FZD8 (frizzled class receptor 8) is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. FZD8 is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined.

Molecular Weight: 75kD

Gene ID: 8325, 7855

UniProt: Q13467, Q9H461

Pathways: WNT Signaling