GPR31 antibody (AA 150-230)

Catalog No. ABIN7221238

This Rabbit Polyclonal antibody specifically detects GPR31 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for GPR31 antibody (AA 150-230) (ABIN7221238)

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GPR31 Antibody

Binding Specificity: AA 150-230

Purpose: Rabbit Anti-GPR31 Polyclonal Antibody

Specificity: GPR31 Polyclonal Antibody detects endogenous levels of GPR31 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human GPR31 at AA range: 150-230

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GPR31

Target: GPR31 (G Protein-Coupled Receptor 31 (GPR31))

Alternative Name: GPR31

Background: GPR31, 12-(S)-hydroxy-5, 8,10,14-eicosatetraenoic acid receptor, 12-(S)-HETE receptor, 12-HETER, G-protein coupled receptor 31GPR31 (G Protein-Coupled Receptor 31) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Peptide ligand-binding receptors. GO annotations related to this gene include G-protein coupled receptor activity. An important paralog of this gene is HCAR3. igh-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.

Molecular Weight: 33kD

Gene ID: 2853

UniProt: O00270