GRK1 antibody (pSer21)

Catalog No. ABIN7224022

The Rabbit Polyclonal anti-GRK1 antibody (ABIN7224022) specifically detects GRK1 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for GRK1 antibody (pSer21) (ABIN7224022)

Target: GRK1 (G Protein-Coupled Receptor Kinase 1 (GRK1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-GRK1 Antibody

Binding Specificity: pSer21

Purpose: Rabbit Anti-GRK 1 (phospho Ser21) Polyclonal Antibody

Specificity: Phospho-GRK 1 (S21) Polyclonal Antibody detects endogenous levels of GRK 1 protein only when phosphorylated at S21.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human GRK 1 Phospho-Ser21

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GRK1

Target: GRK1 (G Protein-Coupled Receptor Kinase 1 (GRK1))

Alternative Name: GRK 1

Background: GRK1, RHOK, Rhodopsin kinase, RK, G protein-coupled receptor kinase 1GRK1 (G protein-coupled receptor kinase 1) encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2).

Molecular Weight: 63kD

Gene ID: 6011

UniProt: Q15835

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction