GALR2 antibody (AA 180-260)

Catalog No. ABIN7225664

The Rabbit Polyclonal anti-GALR2 antibody is suitable to detect GALR2 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for GALR2 antibody (AA 180-260) (ABIN7225664)

Target: GALR2 (Galanin Receptor 2 (GALR2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALR2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GALR2 Antibody

Binding Specificity: AA 180-260

Purpose: Rabbit Anti-GALR2 Polyclonal Antibody

Specificity: GALR2 Polyclonal Antibody detects endogenous levels of GALR2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human GALR2 at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GALR2

Target: GALR2 (Galanin Receptor 2 (GALR2))

Alternative Name: GALR2

Background: GALR2, GALNR2, Galanin receptor type 2, GAL2-R, GALR-2Galanin is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamopituitary axis. It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The actions of galanin are mediated through interaction with specific membrane receptors that are members of the 7-transmembrane family of G protein-coupled receptors. GALR2 interacts with the N-terminal residues of the galanin peptide. The primary signaling mechanism for GALR2 is through the phospholipase C/protein kinase C pathway (via Gq), in contrast to GALR1, which communicates its intracellular signal by inhibition of adenylyl cyclase through Gi. However, it has been demonstrated that GALR2 couples efficiently to both the Gq and Gi proteins to simultaneously activate 2 independent signal transduction pathways.

Molecular Weight: 42kD

Gene ID: 8811

UniProt: O43603

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Feeding Behaviour