GATA1 antibody

Catalog No. ABIN7215010

The Rabbit Polyclonal anti-GATA1 antibody has been validated for WB, ELISA, IP, IF and IHC (p). It is suitable to detect GATA1 in samples from Human, Mouse and Rat.

Quick Overview for GATA1 antibody (ABIN7215010)

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GATA1 Antibody

Purpose: GATA-1 Polyclonal Antibody

Specificity: GATA-1 Polyclonal Antibody detects endogenous levels of GATA-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human GATA-1 around the non-phosphorylation site of S142

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IP (2-5 μg/mg lysate), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GATA1

Target: GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))

Alternative Name: GATA-1

Background: Rabbit Anti-GATA-1 Polyclonal Antibody,GATA1, ERYF1, GF1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, GATA-1, GF-1, NF-E1 DNA-binding protein,GATA1 encodes Erythroid transcription factor which belongs to the GATA family of transcription factors. Erythroid transcription factor plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in GATA1 have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.,Erythroid transcription factor

Molecular Weight: observerd band 43kDa

Gene ID: 2623

UniProt: P15976

Pathways: Cellular Response to Molecule of Bacterial Origin