GTF3A antibody (Internal Region)

Catalog No. ABIN7221396

This Rabbit Polyclonal antibody specifically detects GTF3A in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GTF3A antibody (Internal Region) (ABIN7221396)

Target: GTF3A (General Transcription Factor IIIA (GTF3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GTF3A Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-TFIIIA Polyclonal Antibody

Specificity: TFIIIA Polyclonal Antibody detects endogenous levels of TFIIIA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human TFIIIA

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GTF3A

Target: GTF3A (General Transcription Factor IIIA (GTF3A))

Alternative Name: TFIIIA

Background: GTF3A, Transcription factor IIIA, TFIIIAThe product of GTF3A is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein.

Molecular Weight: 41kD

Gene ID: 2971

UniProt: Q92664