GCK antibody (AA 221-270)

Catalog No. ABIN7228630

This Rabbit Polyclonal antibody specifically detects GCK in WB and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for GCK antibody (AA 221-270) (ABIN7228630)

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GCK Antibody

Binding Specificity: AA 221-270

Purpose: GCK/GLK Polyclonal Antibody

Specificity: The antibody detects endogenous levels of GCK/GLK

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human GCK/GLK protein at AA range: 221-270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: GCK/GLK

Background: Rabbit Anti-GCK/GLK Polyclonal Antibody,Mitogen-activated protein kinase kinase kinase kinase 2, B lymphocyte serine/threonine-protein kinase, Germinal center kinase, GC kinase, MAPK/ERK kinase kinase kinase 2, MEK kinase kinase 2, MEKKK 2, Rab8-interacting protein Mitogen-activated protein kinase kinase kinase kinase 3, Germinal center kinase-related protein kinase, GLK, MAPK/ERK kinase kinase kinase 3, MEK kinase kinase 3, MEKKK 3,The protein encoded by MAP4K2 is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. Alternative splicing results in multiple transcript variants.,GCK/GLK

Molecular Weight: observerd band 100kDa

Gene ID: 5871, 8491

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process