GPT2 antibody (AA 190-270)

Catalog No. ABIN7225458

The Rabbit Polyclonal anti-GPT2 antibody is suitable to detect GPT2 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for GPT2 antibody (AA 190-270) (ABIN7225458)

Target: GPT2 (Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GPT2 Antibody

Binding Specificity: AA 190-270

Purpose: Rabbit Anti-GPT2 Polyclonal Antibody

Specificity: GPT2 Polyclonal Antibody detects endogenous levels of GPT2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human GPT2 at AA range: 190-270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GPT2

Target: GPT2 (Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2))

Alternative Name: GPT2

Background: GPT2, AAT2, ALT2, Alanine aminotransferase 2, ALT2, Glutamate pyruvate transaminase 2, GPT 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2GPT2 (glutamic--pyruvic transaminase 2) encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in GPT2 has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants.

Molecular Weight: 50kD

Gene ID: 84706

UniProt: Q8TD30