GP1BB antibody (AA 90-150)

Catalog No. ABIN7227754

The Rabbit Polyclonal anti-GP1BB antibody is suitable to detect GP1BB in samples from Human, Rat and Mouse. It has been validated for IHC, IF and ELISA.

Quick Overview for GP1BB antibody (AA 90-150) (ABIN7227754)

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GP1BB antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-GP1BB Antibody

Binding Specificity: AA 90-150

Purpose: Rabbit Anti-CD42c Polyclonal Antibody

Specificity: The antibody detects endogenous levels of CD42c

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human CD42c protein at AA range: 90-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:50-200,ELISA 1:10000-20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GP1BB

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Alternative Name: CD42c

Background: Platelet glycoprotein Ib beta chain, GP-Ib beta, GPIb-beta, GPIbB, Antigen CD42b-beta, CD antigen CD42cPlatelet glycoprotein Ib (GPIb) is a heterodimeric transmembrane protein consisting of a disulfide-linked 140 kD alpha chain and 22 kD beta chain. It is part of the GPIb-V-IX system that constitutes the receptor for von Willebrand factor (VWF), and mediates platelet adhesion in the arterial circulation. GPIb alpha chain provides the VWF binding site, and GPIb beta contributes to surface expression of the receptor and participates in transmembrane signaling through phosphorylation of its intracellular domain. Mutations in the GPIb beta subunit have been associated with Bernard-Soulier syndrome, velocardiofacial syndrome and giant platelet disorder. The 206 amino acid precursor of GPIb beta is synthesized from a 1. kb mRNA expressed in plateletes and megakaryocytes. A 411 amino acid protein arising from a longer, unspliced transcript in endothelial cells has been described, however, the authenticity of this product has been questioned. Yet another less abundant GPIb beta mRNA species of 3. kb, expressed in nonhematopoietic tissues such as endothelium, brain and heart, was shown to result from inefficient usage of a non-consensus polyA signal in the neighboring upstream gene (SEPT5, septin 5). In the absence of polyadenylation from its own imperfect site, the SEPT5 gene produces read-through transcripts that use the consensus polyA signal of GP1BB.

Gene ID: 2812

UniProt: P13224