GNAI3 antibody (AA 1-80)

Catalog No. ABIN7221160

The Rabbit Polyclonal anti-GNAI3 antibody is suitable to detect GNAI3 in samples from Human, Rat and Mouse. It has been validated for WB and ELISA.

Quick Overview for GNAI3 antibody (AA 1-80) (ABIN7221160)

Target: GNAI3 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 3 (GNAI3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GNAI3 Antibody

Binding Specificity: AA 1-80

Purpose: Gα i-3 Polyclonal Antibody

Specificity: Gα i-3 Polyclonal Antibody detects endogenous levels of Gα i-3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Galpha i-3 at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for GNAI3

Target: GNAI3 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 3 (GNAI3))

Alternative Name: Galpha i-3

Background: Rabbit Anti-Gα i-3 Polyclonal Antibody,GNAI3, Guanine nucleotide-binding protein G(k) subunit alpha, G(i) alpha-3,Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. GNAI3 encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.,Guanine nucleotide-binding protein G(k) subunit alpha

Molecular Weight: observerd band 41kDa

Gene ID: 2773

UniProt: P08754

Pathways: cAMP Metabolic Process, G-protein mediated Events