Nucleostemin antibody

Catalog No. ABIN7228696

The Rabbit Polyclonal anti-Nucleostemin antibody is suitable to detect Nucleostemin in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for Nucleostemin antibody (ABIN7228696)

Target: Nucleostemin (GNL3) (Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleostemin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Nucleostemin Antibody

Purpose: Rabbit Anti-GNL3 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of GNL3 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human GNL3 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Nucleostemin

Target: Nucleostemin (GNL3) (Guanine Nucleotide Binding Protein-Like 3 (Nucleolar) (GNL3))

Alternative Name: GNL3

Background: Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, NucleosteminThe protein encoded by GNL3 may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for GNL3. GNL3 (G Protein Nucleolar 3) is a Protein Coding gene. Among its related pathways are Ribosome biogenesis in eukaryotes and Neural Stem Cell Differentiation Pathways and Lineage-specific Markers.

Molecular Weight: 60kD

Gene ID: 26354

UniProt: Q9BVP2