GUF1 antibody (AA 390-470)

Catalog No. ABIN7224038

This Rabbit Polyclonal antibody specifically detects GUF1 in ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for GUF1 antibody (AA 390-470) (ABIN7224038)

Target: GUF1 (GUF1 GTPase Homolog (GUF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GUF1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GUF1 Antibody

Binding Specificity: AA 390-470

Purpose: Rabbit Anti-GUF1 Polyclonal Antibody

Specificity: GUF1 Polyclonal Antibody detects endogenous levels of GUF1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human GUF1 at AA range: 390-470

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GUF1

Target: GUF1 (GUF1 GTPase Homolog (GUF1))

Alternative Name: GUF1

Background: GUF1, Translation factor GUF1, mitochondrial, Elongation factor 4 homolog, EF-4, GTPase GUF1, Ribosomal back-translocaseGUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.

Gene ID: 60558

UniProt: Q8N442