HSP27 antibody (pSer15)

Catalog No. ABIN7212992

This anti-HSP27 antibody is a Rabbit Polyclonal antibody detecting HSP27 in WB, ELISA and IHC (p). Suitable for Human and Monkey.

Quick Overview for HSP27 antibody (pSer15) (ABIN7212992)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HSP27 Antibody

Binding Specificity: pSer15

Purpose: HSP27 (phospho Ser15) Polyclonal Antibody

Specificity: Phospho-HSP27 (S15) Polyclonal Antibody detects endogenous levels of HSP27 protein only when phosphorylated at S15.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human HSP27 Phospho-Ser15

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSP27

Background: Rabbit Anti-HSP27 (phospho Ser15) Polyclonal Antibody,HSPB1, HSP27, HSP28, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27,Heat shock protein family B (small) member 1 encoded by HSPB1 is induced by environmental stress and developmental changes. Heat shock protein family B (small) member 1 is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in HSPB1 are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).,Heat shock protein beta-1

Molecular Weight: observerd band 26kDa

Gene ID: 3315

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling