HSF1 antibody (pSer303)

Catalog No. ABIN7221572

This Rabbit Polyclonal antibody specifically detects HSF1 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for HSF1 antibody (pSer303) (ABIN7221572)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HSF1 Antibody

Binding Specificity: pSer303

Purpose: HSF1 (phospho Ser303) Polyclonal Antibody

Specificity: Phospho-HSF1 (S303) Polyclonal Antibody detects endogenous levels of HSF1 protein only when phosphorylated at S303.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human HSF1 Phospho-Ser303

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background: Rabbit Anti-HSF1 (phospho Ser303) Polyclonal Antibody,HSF1, HSTF1, Heat shock factor protein 1, HSF 1, Heat shock transcription factor 1, HSTF 1,The product of HSF1 is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1.,Heat shock factor protein 1

Molecular Weight: observerd band 82kDa

Gene ID: 3297

UniProt: Q00613