His Tag antibody

Catalog No. ABIN7212544

This anti-His Tag antibody is a Rabbit Polyclonal antibody detecting His Tag in WB and ELISA. Suitable for not_set.

Quick Overview for His Tag antibody (ABIN7212544)

Target: His Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-His Tag Antibody

Purpose: His-tag Polyclonal Antibody

Specificity: His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: 6X His synthetic peptide conjugated to KLH

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:3000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.05 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for His Tag

Target: His Tag

Alternative Name: His-tag

Target Type: Tag

Background: Rabbit Anti-His-tag Polyclonal Antibody,His, 6 His epitope, Hexa His, HHHHHH epitope, Polyhistidine Tag,The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein's structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.,His

Molecular Weight: observerd band 15kDa