HDAC4 antibody

Catalog No. ABIN7215208

The Rabbit Polyclonal anti-HDAC4 antibody is suitable to detect HDAC4 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for HDAC4 antibody (ABIN7215208)

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HDAC4 Antibody

Purpose: Rabbit Anti-HDAC4 Polyclonal Antibody

Specificity: HDAC4 Polyclonal Antibody detects endogenous levels of HDAC4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human HDAC4 around the non-phosphorylation site of S632

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HDAC4

Target: HDAC4 (Histone Deacetylase 4 (HDAC4))

Alternative Name: HDAC4

Background: HDAC4, KIAA0288, Histone deacetylase 4, HD4Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase 4 encoded by HDAC4 belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. Histone deacetylase 4 does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Molecular Weight: 119kD

Gene ID: 9759

UniProt: P56524

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process