HDAC5 antibody (AA 1050-1130)

Catalog No. ABIN7216150

The Rabbit Polyclonal anti-HDAC5 antibody is suitable to detect HDAC5 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for HDAC5 antibody (AA 1050-1130) (ABIN7216150)

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-HDAC5 Antibody

Binding Specificity: AA 1050-1130

Purpose: Rabbit Anti-NY-CO-9 Polyclonal Antibody

Specificity: NY-CO-9 Polyclonal Antibody detects endogenous levels of NY-CO-9 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human NY-CO-9 at AA range: 1050-1130

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Alternative Name: NY-CO-9

Background: HDAC5, KIAA0600, Histone deacetylase 5, HD5, Antigen NY-CO-9Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. Histone deacetylase 5 by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. HDAC5 is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for HDAC5.

Molecular Weight: 121kD

Gene ID: 10014

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process