Histone H4 antibody (H4K21me1)

Catalog No. ABIN7229724

This Rabbit Polyclonal antibody specifically detects Histone H4 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Histone H4 antibody (H4K21me1) (ABIN7229724)

Target: Histone H4 (HISTH4)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Histone H4 Antibody

Binding Specificity: H4K21me1

Purpose: Rabbit Anti-Mono-Methyl-Histone H4 (K21) Polyclonal Antibody

Specificity: The antibody detects endogenous levels of Mono-Methyl-Histone H4 (K21)

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human Mono-Methyl-Histone H4 (K21) protein at AA range: 21

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:10000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Histone H4

Target: Histone H4 (HISTH4)

Alternative Name: Histone H4

Background: Histone H4Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST4H4 is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from HIST4H4 lack polyA tails, instead, they contain a palindromic termination element.

Molecular Weight: 11kD

Gene ID: 121504

UniProt: P62805