HNF4 alpha/gamma antibody (acLys127)

Catalog No. ABIN7228950

This Rabbit Polyclonal antibody specifically detects HNF4 alpha/gamma in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HNF4 alpha/gamma antibody (acLys127) (ABIN7228950)

Target: HNF4 alpha/gamma

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF4 alpha/gamma antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HNF4 alpha/gamma Antibody

Binding Specificity: acLys127

Purpose: Rabbit Anti-HNF-4α/γ (Acetyl Lys127/79) Polyclonal Antibody

Specificity: The antibody detects endogenous levels of HNF-4α/γ when Acetyl occurs at Lys127/79(human/mouse) or Lys127(rat).

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human HNF-4alpha/gamma (Acetyl Lys127/79) protein at AA range: 127 (HNF-4alpha) /79 (HNF-4gamma)

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:10000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HNF4 alpha/gamma

Target: HNF4 alpha/gamma

Alternative Name: HNF-4alpha/gamma

Background: Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14 Hepatocyte nuclear factor 4-gamma, HNF-4-gamma, Nuclear receptor subfamily 2 group A member 2(TCF-14, Transcription factor HNF-4The protein encoded by HNF4A is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. HNF4A may play a role in development of the liver, kidney, and intestines. Mutations in HNF4A have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of HNF4A results in multiple transcript variants encoding several different isoforms. HNF4A (Hepatocyte Nuclear Factor 4 Alpha) is a Protein Coding gene. Diseases associated with HNF4A include Mody, Type I and Fanconi Renotubular Syndrome 4, With Maturity-Onset Diabetes Of The Young. Among its related pathways are Type II diabetes mellitus and Mesodermal Commitment Pathway.

Molecular Weight: 55kD

Gene ID: 3172

UniProt: P41235, Q14541