HNF4 alpha/gamma antibody (acLys79, acLys127)

Catalog No. ABIN7228950

This Rabbit Polyclonal antibody specifically detects HNF4 alpha/gamma in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HNF4 alpha/gamma antibody (acLys79, acLys127) (ABIN7228950)

Target: HNF4 alpha/gamma

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNF4 alpha/gamma antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HNF4 alpha/gamma Antibody

Binding Specificity: acLys79, acLys127

Purpose: HNF-4α/γ (Acetyl Lys127/79) Polyclonal Antibody

Specificity: The antibody detects endogenous levels of HNF-4α/γ when Acetyl occurs at Lys127/79(human/mouse) or Lys127(rat).

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human HNF-4alpha/gamma (Acetyl Lys127/79) protein at AA range: 127 (HNF-4alpha) /79 (HNF-4gamma)

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for HNF4 alpha/gamma

Target: HNF4 alpha/gamma

Alternative Name: HNF-4alpha/gamma

Background: Rabbit Anti-HNF-4α/γ (Acetyl Lys127/79) Polyclonal Antibody,Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14 Hepatocyte nuclear factor 4-gamma, HNF-4-gamma, Nuclear receptor subfamily 2 group A member 2(TCF-14, Transcription factor HNF-4,The protein encoded by HNF4A is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. HNF4A may play a role in development of the liver, kidney, and intestines. Mutations in HNF4A have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of HNF4A results in multiple transcript variants encoding several different isoforms. HNF4A (Hepatocyte Nuclear Factor 4 Alpha) is a Protein Coding gene. Diseases associated with HNF4A include Mody, Type I and Fanconi Renotubular Syndrome 4, With Maturity-Onset Diabetes Of The Young. Among its related pathways are Type II diabetes mellitus and Mesodermal Commitment Pathway.,HNF-4α/γ

Molecular Weight: observerd band 55kDa

Gene ID: 3172