HCFC1 antibody (AA 100-180)

Catalog No. ABIN7221454

The Rabbit Polyclonal anti-HCFC1 antibody is suitable to detect HCFC1 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for HCFC1 antibody (AA 100-180) (ABIN7221454)

Target: HCFC1 (Host Cell Factor C1 (VP16-Accessory Protein) (HCFC1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCFC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-HCFC1 Antibody

Binding Specificity: AA 100-180

Purpose: Rabbit Anti-HCF1 Polyclonal Antibody

Specificity: HCF1 Polyclonal Antibody detects endogenous levels of HCF1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human HCF1 at AA range: 100-180

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HCFC1

Target: HCFC1 (Host Cell Factor C1 (VP16-Accessory Protein) (HCFC1))

Alternative Name: HCF1

Background: HCFC1, HCF1, HFC1, Host cell factor 1, HCF, HCF-1, C1 factor, CFF, VCAF, VP16 accessory proteinHCFC1 is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described, however, not all variants have been fully characterized.

Molecular Weight: 208kD

Gene ID: 3054

UniProt: P51610