HUS1 antibody (AA 160-240)

Catalog No. ABIN7228986

The Rabbit Polyclonal anti-HUS1 antibody (ABIN7228986) specifically detects HUS1 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for HUS1 antibody (AA 160-240) (ABIN7228986)

Target: HUS1 (HUS1 Checkpoint Homolog (S. Pombe) (HUS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HUS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HUS1 Antibody

Binding Specificity: AA 160-240

Purpose: HUS1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of HUS1 protein

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human HUS1 protein at AA range: 160-240

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for HUS1

Target: HUS1 (HUS1 Checkpoint Homolog (S. Pombe) (HUS1))

Alternative Name: HUS1

Background: Rabbit Anti-HUS1 Polyclonal Antibody,Checkpoint protein HUS1, hHUS1,The protein encoded by HUS1 is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.,HUS1

Molecular Weight: observerd band 30kDa

Gene ID: 3364

UniProt: O60921