IKK-alpha /IKK-beta antibody (pSer180, pSer181)

Catalog No. ABIN7213006

The Rabbit Polyclonal anti-IKK-alpha /IKK-beta antibody is suitable to detect IKK-alpha /IKK-beta in samples from Mouse, Human and Rat. It has been validated for WB, ELISA and IHC (p).

Quick Overview for IKK-alpha /IKK-beta antibody (pSer180, pSer181) (ABIN7213006)

Target: IKK-alpha /IKK-beta

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK-alpha /IKK-beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IKK-alpha /IKK-beta Antibody

Binding Specificity: pSer180, pSer181

Purpose: IKKα/β (phospho Ser180/181) Polyclonal Antibody

Specificity: Phospho-IKKα/β (S180/181) Polyclonal Antibody detects endogenous levels of IKKα/β protein only when phosphorylated at S180/181.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human IKKalpha/beta Phospho-Ser180/181

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for IKK-alpha /IKK-beta

Target: IKK-alpha /IKK-beta

Alternative Name: IKKalpha/beta

Background: Rabbit Anti-IKKα/β (phospho Ser180/181) Polyclonal Antibody,CHUK, IKKA, TCF16, Inhibitor of nuclear factor kappa-B kinase subunit alpha, I-kappa-B kinase alpha, IKK-A, IKK-alpha, IkBKA, IkappaB kinase, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase 1, IKK1, Nuclear factor NF-kappa-B,CHUK encodes a member of the serine/threonine protein kinase family. Conserved helix-loop-helix ubiquitous kinase, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.,Inhibitor of nuclear factor kappa-B kinase subunit alpha

Molecular Weight: observerd band 85kDa

Gene ID: 1147, 3551