Integrin beta 4 antibody (pTyr1510)

Catalog No. ABIN7221910

This Rabbit Polyclonal antibody specifically detects Integrin beta 4 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Integrin beta 4 antibody (pTyr1510) (ABIN7221910)

Target: Integrin beta 4 (ITGB4)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Integrin beta 4 Antibody

Binding Specificity: pTyr1510

Purpose: Rabbit Anti-Integrin β4 (phospho Tyr1510) Polyclonal Antibody

Specificity: Phospho-Integrin β4 (Y1510) Polyclonal Antibody detects endogenous levels of Integrin β4 protein only when phosphorylated at Y1510.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Integrin beta4 Phospho-Tyr1510

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: Integrin beta4

Background: ITGB4, Integrin beta-4, GP150, CD antigen CD104Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. ITGB4 encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molecular Weight: 202kD

Gene ID: 3691

UniProt: P16144

Pathways: Integrin Complex