SH3KBP1 antibody (AA 591-665) (FITC)

Catalog No. ABIN716223

This anti-SH3KBP1 antibody is a Rabbit Polyclonal antibody detecting SH3KBP1 in WB, IF (cc) and IF (p). Suitable for Human.

Quick Overview for SH3KBP1 antibody (AA 591-665) (FITC) (ABIN716223)

Target: SH3KBP1 (SH3-Domain Kinase Binding Protein 1 (SH3KBP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH3KBP1 antibody is conjugated to FITC

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-SH3KBP1 Antibody (FITC)

Binding Specificity: AA 591-665

Predicted Reactivity: Human,Mouse,Rat,Cow,Sheep,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SH3KBP1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SH3KBP1

Target: SH3KBP1 (SH3-Domain Kinase Binding Protein 1 (SH3KBP1))

Alternative Name: SH3KBP1

Background:

Synonyms: Cbl interacting protein, Cbl interacting protein of 85 kDa, CD2 binding protein 3, CD2BP3, CIN 85, CIN85, GIG 10, GIG10, HSB 1, Human Src family kinase binding protein 1, MIG 18, MIG18, Migration inducing gene 18 protein, SH3 domain kinase binding protein 1, SH3BP 1, Src family kinase binding protein 1, src related kinase binding protein 1, SH3K1_HUMAN.

Background: SH3KBP1 belongs to the CIN85/CMS family of adaptor molecules, characterized by containing three N-terminal Src homology domains, a proline rich region and a C-terminal coiled-coil domain. The different members of the family orchestrate a network involved in dowregulation and degradation of recpetor tyrosine kinases. SH3KBP1 is involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. In the case of EGF receptor turnover, its activation involves recruitment of SH3KBP1- endophilin complexes to mediate internalization. Once internalized, RTKs are delivered into the endosomal compartment where receptors get sorted for either recycling back to the cell surface or are targeted to lysosomes for degradation. Alternate splicing results in multiple transcript variants.

Gene ID: 30011

Pathways: EGFR Signaling Pathway, EGFR Downregulation