ISG15 antibody

Catalog No. ABIN7229190

The Rabbit Polyclonal anti-ISG15 antibody is suitable to detect ISG15 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for ISG15 antibody (ABIN7229190)

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ISG15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ISG15 Antibody

Purpose: Rabbit Anti-ISG15 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of ISG15 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human ISG15 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ISG15

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Alternative Name: ISG15

Background: Ubiquitin-like protein ISG15, Interferon-induced 15 kDa protein, Interferon-induced 17 kDa protein, IP17, Ubiquitin cross-reactive protein, hUCRPISG15 Ubiquitin-Like Modifier encoded by ISG15 is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, ISG15 Ubiquitin-Like Modifier is sometimes secreted. ISG15 (ISG15 Ubiquitin-Like Modifier) is a Protein Coding gene. Diseases associated with ISG15 include Immunodeficiency 38 and Influenza. Among its related pathways are Type II interferon signaling (IFNG) and Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template.

Molecular Weight: 18kD

Gene ID: 9636

UniProt: P05161