JAK2 antibody (pTyr119)

Catalog No. ABIN7221940

The Rabbit Polyclonal anti-JAK2 antibody has been validated for ELISA and IHC (p). It is suitable to detect JAK2 in samples from Human, Rat and Mouse.

Quick Overview for JAK2 antibody (pTyr119) (ABIN7221940)

Target: JAK2 (Janus Kinase 2 (JAK2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-JAK2 Antibody

Binding Specificity: pTyr119

Purpose: JAK2 (phospho Tyr119) Polyclonal Antibody

Specificity: Phospho-JAK2 (Y119) Polyclonal Antibody detects endogenous levels of JAK2 protein only when phosphorylated at Y119.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human JAK2 Phospho-Tyr119

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Alternative Name: JAK2

Background: Rabbit Anti-JAK2 (phospho Tyr119) Polyclonal Antibody,JAK2, Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2,JAK2 product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis.,Tyrosine-protein kinase JAK2

Molecular Weight: observerd band 130kDa

Gene ID: 3717

UniProt: O60674

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response