KSR1 antibody (pSer392)

Catalog No. ABIN7213238

This Rabbit Polyclonal antibody specifically detects KSR1 in WB, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for KSR1 antibody (pSer392) (ABIN7213238)

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KSR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KSR1 Antibody

Binding Specificity: pSer392

Purpose: Ksr-1 (phospho Ser392) Polyclonal Antibody

Specificity: Phospho-Ksr-1 (S392) Polyclonal Antibody detects endogenous levels of Ksr-1 protein only when phosphorylated at S392.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Ksr-1 Phospho-Ser392

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for KSR1

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Alternative Name: Ksr-1

Background: Rabbit Anti-Ksr-1 (phospho Ser392) Polyclonal Antibody,KSR1, KSR, Kinase suppressor of Ras 1,Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 has intrinsic kinase activity towards serine/threonine residues and is widely expressed in many tissue types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood. Two proteins putatively involved in this process are Ksr-1 and Tak1. Ksr-1 (kinase suppressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known. Tak1 (TGFβ-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFβ stimulation.,Kinase suppressor of Ras 1

Molecular Weight: observerd band 115kDa

Gene ID: 8844

UniProt: Q8IVT5