L1CAM antibody

Catalog No. ABIN7215972

The Rabbit Polyclonal anti-L1CAM antibody is suitable to detect L1CAM in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for L1CAM antibody (ABIN7215972)

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This L1CAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-L1CAM Antibody

Purpose: Rabbit Anti-NCAM-L1 Polyclonal Antibody

Specificity: NCAM-L1 Polyclonal Antibody detects endogenous levels of NCAM-L1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human NCAM-L1 around the non-phosphorylation site of S1181

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for L1CAM

Target: L1CAM (L1 Cell Adhesion Molecule (L1CAM))

Alternative Name: NCAM-L1

Background: L1CAM, CAML1, MIC5, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1, CD antigen CD171Neural cell adhesion molecule L1 encoded by L1CAM is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.

Molecular Weight: 180kD

Gene ID: 3897

UniProt: P32004

Pathways: Synaptic Membrane