LAMC3 antibody (AA 1330-1410)

Catalog No. ABIN7218964

This Rabbit Polyclonal antibody specifically detects LAMC3 in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Mouse and Human.

Quick Overview for LAMC3 antibody (AA 1330-1410) (ABIN7218964)

Target: LAMC3 (Laminin, gamma 3 (LAMC3))

Reactivity: Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAMC3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-LAMC3 Antibody

Binding Specificity: AA 1330-1410

Purpose: Laminin γ-3 Polyclonal Antibody

Specificity: Laminin γ-3 Polyclonal Antibody detects endogenous levels of Laminin γ-3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Laminin gamma-3 at AA range: 1330-1410

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for LAMC3

Target: LAMC3 (Laminin, gamma 3 (LAMC3))

Alternative Name: Laminin gamma-3

Background: Rabbit Anti-Laminin γ-3 Polyclonal Antibody,LAMC3, Laminin subunit gamma-3, Laminin-12 subunit gamma, Laminin-14 subunit gamma, Laminin-15 subunit gamma,Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. Alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. LAMC3 encodes the gamma chain isoform laminin, gamma 3. The gamma 3 chain is most similar to the gamma 1 chain, and contains all the 6 domains expected of the gamma chain. It is a component of laminin 12. The gamma 3 chain is broadly expressed in skin, heart, lung, and the reproductive tracts. In skin, it is seen within the basement membrane of the dermal-epidermal junction at points of nerve penetration. Gamma 3 is also a prominent element of the apical surface of ciliated epithelial cells of lung, oviduct, epididymis, ductus deferens, and seminiferous tubules. The distribution of gamma 3-containing laminins along ciliated epithelial surfaces suggests that the apical laminins are important in the morphogenesis and structural stability of the ciliated processes of these cells.,Laminin subunit gamma-3

Molecular Weight: observerd band 170kDa

Gene ID: 10319

UniProt: Q9Y6N6