Latrophilin 1 antibody (AA 530-610)

Catalog No. ABIN7220562

The Rabbit Polyclonal anti-Latrophilin 1 antibody is suitable to detect Latrophilin 1 in samples from Rat, Human and Mouse. It has been validated for ELISA, WB and IHC.

Quick Overview for Latrophilin 1 antibody (AA 530-610) (ABIN7220562)

Target: Latrophilin 1 (LPHN1)

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Latrophilin 1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Latrophilin 1 Antibody

Binding Specificity: AA 530-610

Purpose: Rabbit Anti-Latrophilin-1 Polyclonal Antibody

Specificity: Latrophilin-1 Polyclonal Antibody detects endogenous levels of Latrophilin-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Latrophilin-1 at AA range: 530-610

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Latrophilin 1

Target: Latrophilin 1 (LPHN1)

Alternative Name: Latrophilin-1

Background: LPHN1, KIAA0821, LEC2, Latrophilin-1, Calcium-independent alpha-latrotoxin receptor 1, CIRL-1, Lectomedin-2ADGRL1 encodes a member of the latrophilin subfamily of G-protein coupled receptors (GPCR). Latrophilins may function in both cell adhesion and signal transduction. In experiments with non-human species, endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resulted in two subunits (a large extracellular N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. Latrophilin-1 has been shown to recruit the neurotoxin from black widow spider venom, alpha-latrotoxin, to the synapse plasma membrane. Alternative splicing results in multiple variants encoding distinct isoforms.

Gene ID: 22859

UniProt: O94910

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis