Leptin Receptor antibody (pTyr1141)

Catalog No. ABIN7213352

The Rabbit Polyclonal anti-Leptin Receptor antibody has been validated for WB and ELISA. It is suitable to detect Leptin Receptor in samples from Human and Mouse.

Quick Overview for Leptin Receptor antibody (pTyr1141) (ABIN7213352)

Target: Leptin Receptor (LEPR)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Leptin Receptor Antibody

Binding Specificity: pTyr1141

Purpose: Ob-R (phospho Tyr1141) Polyclonal Antibody

Specificity: Phospho-Ob-R (Y1141) Polyclonal Antibody detects endogenous levels of Ob-R protein only when phosphorylated at Y1141.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Ob-R Phospho-Tyr1141

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Leptin Receptor

Target: Leptin Receptor (LEPR)

Alternative Name: Ob-R

Background: Rabbit Anti-Ob-R (phospho Tyr1141) Polyclonal Antibody,LEPR, DB, OBR, Leptin receptor, LEP-R, HuB219, OB receptor, OB-R, CD antigen CD295,Leptin receptor encoded by LEPR belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. Leptin receptor is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in LEPR have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for LEPR. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).,Leptin receptor

Molecular Weight: observerd band 132kDa

Gene ID: 3953

UniProt: P48357

Pathways: JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour