LIM Domain Kinase 1 antibody (pThr508)

Catalog No. ABIN7213028

The Rabbit Polyclonal anti-LIM Domain Kinase 1 antibody is suitable to detect LIM Domain Kinase 1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for LIM Domain Kinase 1 antibody (pThr508) (ABIN7213028)

Target: LIM Domain Kinase 1 (LIMK1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIM Domain Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-LIM Domain Kinase 1 Antibody

Binding Specificity: pThr508

Purpose: Rabbit Anti-LIMK-1 (phospho Thr508) Polyclonal Antibody

Specificity: Phospho-LIMK-1 (T508) Polyclonal Antibody detects endogenous levels of LIMK-1 protein only when phosphorylated at T508.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human LIMK-1 Phospho-Thr508

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for LIM Domain Kinase 1

Target: LIM Domain Kinase 1 (LIMK1)

Alternative Name: LIMK-1

Background: LIMK1, LIMK, LIM domain kinase 1, LIMK-1There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. LIM domain kinase 1 is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. LIM domain kinase 1 also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 72kD

Gene ID: 3984

UniProt: P53667

Pathways: Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events