Luciferase antibody
Quick Overview for Luciferase antibody (ABIN7212242)
Target
See all Luciferase AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
-
-
Purpose
- Luciferase Mouse Monoclonal Antibody (6B8)
-
Specificity
- Luciferase protein detects endogenous levels of Luciferase.
-
Purification
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
-
Immunogen
- Recombinant Protein of Luciferase of Luciferase
-
Isotype
- IgG1
-
-
anti-Luciferase antibody
Reactivity: Firefly ELISA, ICC, IF (p), IF (cc), IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Luciferase antibodyReactivity: Firefly WB, IF Host: Mouse Monoclonal 2C2 unconjugated
anti-Luciferase antibodyReactivity: Photinus pyralis WB, ELISA, IHC, FM Host: Goat Polyclonal unconjugated
anti-Luciferase antibodyReactivity: Firefly WB, ELISA, IHC Host: Mouse Monoclonal unconjugated
anti-Luciferase antibodyReactivity: Photinus pyralis WB Host: Mouse Monoclonal Luci17 unconjugated
anti-Luciferase (C-Term) antibodyReactivity: Photinus pyralis WB Host: Goat Polyclonal unconjugated
anti-Luciferase (N-Term) antibodyReactivity: Photinus pyralis WB, IHC Host: Mouse Monoclonal Luci17 unconjugated
anti-Luciferase antibodyReactivity: Photinus pyralis WB, ELISA Host: Goat Polyclonal unconjugated
anti-Luciferase antibodyReactivity: Vibrio fischeri WB, ELISA, IHC, IP, DB Host: Rabbit Polyclonal unconjugated
-
-
Application Notes
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:2000), IHC-P (1:100-1:200).
-
Comment
-
Primary Antibody
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1 mg/mL
-
Buffer
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
-
Preservative
- Sodium azide
-
Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Storage
- -20 °C
-
Storage Comment
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
-
-
- Luciferase
-
Background
- Mouse Anti-Luciferase Mouse Monoclonal Antibody (6B8),Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.,Luciferase
-
Molecular Weight
- observerd band 60kDa
-
UniProt
- P08659
Target
-
