MPHOSPH8 antibody (AA 290-370)

Catalog No. ABIN7229744

This Rabbit Polyclonal antibody specifically detects MPHOSPH8 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MPHOSPH8 antibody (AA 290-370) (ABIN7229744)

Target: MPHOSPH8 (M-Phase phosphoprotein 8 (MPHOSPH8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MPHOSPH8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MPHOSPH8 Antibody

Binding Specificity: AA 290-370

Purpose: Rabbit Anti-MPP8 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of MPP8 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human MPP8 protein at AA range: 290-370

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MPHOSPH8

Target: MPHOSPH8 (M-Phase phosphoprotein 8 (MPHOSPH8))

Alternative Name: MPP8

Background: M-phase phosphoprotein 8, Two hybrid-associated protein 3 with RanBPM, Twa3MPHOSPH8 (M-Phase Phosphoprotein 8) is a Protein Coding gene. Heterochromatin component that specifically recognizes and binds methylated Lys-9 of histone H3 and promotes recruitment of proteins that mediate epigenetic repression. Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is probably required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3. Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites, these can be situated within the coding region of the gene. Mediates down-regulation of CDH1 expression.

Molecular Weight: 94kD

Gene ID: 54737

UniProt: Q99549