ME1 antibody (AA 440-520)

Catalog No. ABIN7222418

The Rabbit Polyclonal anti-ME1 antibody (ABIN7222418) specifically detects ME1 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ME1 antibody (AA 440-520) (ABIN7222418)

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ME1 Antibody

Binding Specificity: AA 440-520

Purpose: Rabbit Anti-Malic enzyme 1 Polyclonal Antibody

Specificity: Malic enzyme 1 Polyclonal Antibody detects endogenous levels of Malic enzyme 1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Malic enzyme 1 at AA range: 440-520

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ME1

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Alternative Name: Malic enzyme 1

Background: ME1, NADP-dependent malic enzyme, NADP-ME, Malic enzyme 1ME1 encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for ME1 is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Molecular Weight: 64kD

Gene ID: 4199

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha