MMP14 antibody (Cleaved-Tyr112)

Catalog No. ABIN7212488

The Rabbit Polyclonal anti-MMP14 antibody is suitable to detect MMP14 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for MMP14 antibody (Cleaved-Tyr112) (ABIN7212488)

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MMP14 Antibody

Binding Specificity: AA 70-150, Cleaved-Tyr112

Purpose: Cleaved-MMP-14 (Y112) Polyclonal Antibody

Specificity: Cleaved-MMP-14 (Y112) Polyclonal Antibody detects endogenous levels of fragment of activated MMP-14 protein resulting from cleavage adjacent to Y112.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human MMP-14 at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MMP14

Target: MMP14 (Matrix Metallopeptidase 14 (Membrane-inserted) (MMP14))

Alternative Name: MMP-14

Background: Rabbit Anti-Cleaved-MMP-14 (Y112) Polyclonal Antibody,MMP14, Matrix metalloproteinase-14, MMP-14, MMP-X1, Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1, Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP,Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by MMP14 is a member of the membrane-type MMP (MT-MMP) subfamily, each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. Matrix metallopeptidase 14 activates MMP2 protein, and this activity may be involved in tumor invasion.,Matrix metalloproteinase-14

Molecular Weight: observerd band 53kDa

Gene ID: 4323

UniProt: P50281

Pathways: Autophagy