MDM1 antibody (AA 640-720)

Catalog No. ABIN7223682

The Rabbit Polyclonal anti-MDM1 antibody is suitable to detect MDM1 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for MDM1 antibody (AA 640-720) (ABIN7223682)

Target: MDM1 (Mdm1 Nuclear Protein (MDM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MDM1 Antibody

Binding Specificity: AA 640-720

Purpose: Rabbit Anti-MDM1 Polyclonal Antibody

Specificity: MDM1 Polyclonal Antibody detects endogenous levels of MDM1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human MDM1 at AA range: 640-720

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MDM1

Target: MDM1 (Mdm1 Nuclear Protein (MDM1))

Alternative Name: MDM1

Background: MDM1, Nuclear protein MDM1MDM1 (Mdm1 nuclear protein) encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles, is amplified in the mouse transformed 3T3 cell line, and the encoded protein is able to bind to p53. Alternatively spliced transcript variants encoding different isoforms have been found for MDM1.

Molecular Weight: 80kD

Gene ID: 56890

UniProt: Q8TC05