MED23 antibody (AA 10-90)

Catalog No. ABIN7214346

The Rabbit Polyclonal anti-MED23 antibody has been validated for ELISA, WB, IF and IHC (p). It is suitable to detect MED23 in samples from Human, Rat and Mouse.

Quick Overview for MED23 antibody (AA 10-90) (ABIN7214346)

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED23 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MED23 Antibody

Binding Specificity: AA 10-90

Purpose: CRSP130 Polyclonal Antibody

Specificity: CRSP130 Polyclonal Antibody detects endogenous levels of CRSP130 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human CRSP130 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MED23

Target: MED23 (Mediator Complex Subunit 23 (MED23))

Alternative Name: CRSP130

Background: Rabbit Anti-CRSP130 Polyclonal Antibody,MED23, ARC130, CRSP3, DRIP130, KIAA1216, SUR2, Mediator of RNA polymerase II transcription subunit 23, Activator-recruited cofactor 130 kDa component, ARC130, Cofactor required for Sp1 transcriptional activation subunit 3, CRSP complex subu,The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. Mediator complex subunit 23 encoded by MED23 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein also acts as a metastasis suppressor. Several alternatively spliced transcript variants encoding different isoforms have been described for MED23.,Mediator of RNA polymerase II transcription subunit 23

Molecular Weight: observerd band 150kDa

Gene ID: 9439

UniProt: Q9ULK4

Pathways: Regulation of Lipid Metabolism by PPARalpha