MED27 antibody (AA 90-170)

Catalog No. ABIN7225992

The Rabbit Polyclonal anti-MED27 antibody has been validated for WB and ELISA. It is suitable to detect MED27 in samples from Human and Mouse.

Quick Overview for MED27 antibody (AA 90-170) (ABIN7225992)

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MED27 Antibody

Binding Specificity: AA 90-170

Purpose: CRSP34 Polyclonal Antibody

Specificity: CRSP34 Polyclonal Antibody detects endogenous levels of CRSP34 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human CRSP34 at AA range: 90-170

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MED27

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Alternative Name: CRSP34

Background: Rabbit Anti-CRSP34 Polyclonal Antibody,MED27, CRSP34, CRSP8, Mediator of RNA polymerase II transcription subunit 27, Cofactor required for Sp1 transcriptional activation subunit 8, CRSP complex subunit 8, Mediator complex subunit 27, P37 TRAP/SMCC/PC2 subunit, Transcriptional co,The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by MED27 (mediator complex subunit 27) is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for MED27, and a pseudogene of MED27 is located on the long arm of chromosome 5.,Mediator of RNA polymerase II transcription subunit 27

Molecular Weight: observerd band 35kDa

Gene ID: 9442

UniProt: Q6P2C8

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha